Непродолжительное гипотермическое хранение спермы в жидком состоянии без замораживания является альтернативой криоконсервации в тех случаях,когда жидкий азот...

Data on use of hypothermic sperm storage in ART.

В статье приведены экспериментальные данные о гипотермическом хранении спермы русского осетра Acipenser gueldenstaedtii, сибирского осетра Acipenser baeri и белуги Huso huso в трех...

We studied neural induction and generation of neuroectoderm in the colonies of human parthenogenetic SC cultured in the presence of 5 and 19±2% oxygen. We found that neuroectoderm was more actively generated at high oxygen content. At the same time, the transcription of stem cell pluripotency...

Human parthenogenetic stem cells are derived from the inner cell mass of blastocysts obtained from unfertilized oocytes that have been stimulated to develop without any participation of male gamete. As parthenogenesis does not involve the destruction of a viable human embryo, the derivation and...

We studied the effect of recombinant human activin A on induced neuroectoderm formation in colonies of human parthenogenetic SC in the absence of feeder cells. It was found that pretreatment of human parthenogenetic SC with activin A suppressed subsequent neural induction. Activin A in a...

Developing of an effective approach to sperm storage for several weeks without freezing can solve transportation problem and facilitate planning of medical procedures in ART programs. Solutions containing simple sugars and no salts are apparently the best preservatives for sperm storage at...

Human sperm can be stored without freezing in electrolyte-free solutions of metabolized monosaccharides for at least two weeks at +4 C and effectively recover motility. The highest rate of sperm preservation was found in electrolyte-free solution of glucose, followed by fructose solution, with...

A new technique is presented (The EAPO Pat.No007992. 05.10.2006) allowing to obtain and utilize sterile and biologically safe empty microspheres for efficient cryopreservation of solitary spermatozoa in IVF/ICSI programmes.

The problem of single sperm cryopreservation can be solved by use of the microcontainers that prevent loss during manipulations. Zonae pellucidae of human and mammalian eggs are the best microcontainers, but their use is limited by risk of contamination with infectious and genetical foreign...

Genomic imprinting is one of the most remarkable and important epigenetic phenomena. A biological ban on parthenogenetic and androgenetic development of mammals is an obvious consequence of genomic imprinting. Genomic imprinting defects may cause malformations, clinical syndromes, and tumor...

Cultural conditions, in vitro gametes and early embryos manipulating can be followed by the disorders of genomic imprinting — epigenetic mechanism providing differential expression of certain genes depending on their parental origin during mammalian embryogenesis. Significant number of genetic...

The autosomal recessive gene hairless (hr) is responsible for the complete hairlessness in mice homozygous for this gene. Hair shedding that begins at the age of 10 days is caused by an abnormal cycle of hair follicle development disturbed at the catagen stage. This results in enhanced programmed...

The mutant gene wellhaaring (we) confers the waved coat in mice, which is most pronounced in homozygotes at 10 to 21 days of postnatal development. Abnormal hair growth and structure in the we/we mutant mice results from defective cell differentiation in the inner root sheath of a hair follicle....

Clonal analysis of parthenogenetic chimeric mouse embryos C57B1/6(PG)<-->BALB/c has shown that parthenogenetic cell clones C57BL/6 are present in the brain, liver, and kidneys of 14- and 18-day-old embryos. The content of the parthenogenetic component (PG) in these organs on day 18 was...

This is a review of the data of clonal analysis of developing tissues in parthenogenetic and androgenetic chimeric mice. The time and causes of death of the parthenogenetic and androgenetic cell clones in chimeras are considered. The data obtained suggest that the development of cell clones,...

We studied the distribution of parthenogenetic cell clones in the retinal pigment epithelium and choroid of eyes on serial sections and in the brain, kidneys, and liver by electrophoretic analysis of glucose phosphate isomerase isozymes in 12 mouse chimeras C57BL/6(PG)<-->BALB/c obtained...

The death of diploid parthenogenetic mammalian embryos, specifically mouse embryos, is related to the effects of genomic imprinting. But in mouse chimeras, many parthenogenetic cell clones retain their viability for long periods of development. We obtained 12 chimeric mice by aggregating diploid...